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Preparation and properties of a DNA mismatch- cleavage fusion protein

Reference	E10135
Principal Investigator / Supervisor	Professor Keith R Fox
Co-Investigators / Co-Supervisors	Professor Tom Brown
Institution	University of Southampton
Department	Centre for Biological Sciences
Funding type	Research
Value (£)	143,616
Status	Completed
Type	Research Grant
Start date	01/12/1998
End date	01/12/2001
Duration	36 months

Abstract

The DNA mismatch binding protein MutS will be fused to the nuclease domain of the type IIs restriction enzyme FokI, generating a hybrid protein which recognises and cleaves mismatch-containing DNA fragments. We will optimise the activity of this fusion protein by altering the linker between the two domains and by using truncated version of MutS. This chimeric protein will have many uses in DNA diagnostics and molecular biology and we will examine its use for removing DNA mismatches generated during PCR and for detecting single base mutations.

Summary

unavailable

Committee	Closed Committee - Engineering & Biological Systems (EBS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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