BBSRC Portfolio Analyser
Award details
Lipid bilayer biosensors: development of cell surface display to produce porins with novel affinities
Reference
E10129
Principal Investigator / Supervisor
Professor Jeremy Lakey
Co-Investigators /
Co-Supervisors
Professor Harry Gilbert
Institution
Newcastle University
Department
School of Cell and Molecular Biosciences
Funding type
Research
Value (£)
178,281
Status
Completed
Type
Research Grant
Start date
06/04/1999
End date
06/04/2002
Duration
36 months
Abstract
Porins, by virtue of their channel size, stability, molecular biology and known high resolution structures offer unique opportunities for membrane protein engineering. Recently, we have shown that they can be used as ligand gated switches in supported bilayer biosensors. This project aims to alter their variable loops to enable porins to bind a variety of ligands and therefore be used to detect a range of biological macromolecules and pollutants. The approach will use random mutagenesis, DNA shuffling, error prone PCR and a porin cell surface display library to select for novel binding functions.
Summary
unavailable
Committee
Closed Committee - Engineering & Biological Systems (EBS)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
X - not in an Initiative
Funding Scheme
X – not Funded via a specific Funding Scheme
I accept the
terms and conditions of use
(opens in new window)
export PDF file
back to list
new search