BBSRC Portfolio Analyser
Award details
Probing conformational heterogeneity of lipase enantioselectivity in organic media using chiral active-site inhibitors
Reference
E09881
Principal Investigator / Supervisor
Dr Marie Claire Parker
Co-Investigators /
Co-Supervisors
Professor Nicholas Turner
Institution
University of Glasgow
Department
School of Chemistry
Funding type
Research
Value (£)
111,914
Status
Completed
Type
Research Grant
Start date
11/01/1999
End date
11/01/2001
Duration
24 months
Abstract
The proposal is aimed at developing novel biophysical methods to probe the conformational heterogeneity of lipase active sites. The technique involves using chiral active-site irreversible inhibitors, a combination of electrospray ionisation mass spectrometry (ESI-MS) and catalytic activity-enantioselectivity measurements to determine if the enatioselectivity can be explained by changes to a heterogeneous population of active sites. The study would be carried out in low-water organic media using well-characterised (both structurally and kinetically) lipolytic enzymes that have been shown to be sensitive to their environmental conditions. Changes to the enzyme's environment mediated by e.g. hydration, solvent changes, enzyme pre- treatments would be studied.
Summary
unavailable
Committee
Closed Committee - Engineering & Biological Systems (EBS)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
X - not in an Initiative
Funding Scheme
X – not Funded via a specific Funding Scheme
I accept the
terms and conditions of use
(opens in new window)
export PDF file
back to list
new search