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Investigating protein-ligand interaction by mass spectrometry

Reference	E08254
Principal Investigator / Supervisor	Professor Carol Robinson
Co-Investigators / Co-Supervisors	Professor Sir Christopher Dobson
Institution	University of Oxford
Department	Oxford Chemistry
Funding type	Research
Value (£)	117,997
Status	Completed
Type	Research Grant
Start date	01/05/1998
End date	01/09/2001
Duration	40 months

Abstract

We propose to exploit the use of mass spectrometry in the study of protein - ligand interactions with the overall objective of evaluating the technique as a potential screening method for selecting ligands. This work will involve an in-depth study of the different factors stabilising protein - ligand interactions in solution and in the gas phase of the mass spectrometer. Using protein - ligand systems which are well characterised in solution we will assess the overall sensitivity of the mass spectrometry method, the quality and rapidity of results and pitfalls that may arise in interpretation of data. Furthermore, we intend to examine the conditions which allow us to observe water molecules in the binding site of the protein and, using hydrogen exchange methods, the conformational changes which occur upon ligand binding.

Summary

unavailable

Committee	Closed Committee - Engineering & Biological Systems (EBS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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