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Electrophoretic sample preconcentration and separation applied to direct immunosensors

Reference	E08083
Principal Investigator / Supervisor	Professor Nick Goddard
Co-Investigators / Co-Supervisors	Professor Peter Fielden
Institution	The University of Manchester
Department	Chem Eng and Analytical Science
Funding type	Research
Value (£)	148,484
Status	Completed
Type	Research Grant
Start date	01/04/1997
End date	01/04/2000
Duration	36 months

Abstract

The proposed study is intended to demonstrate the application of iso-electric focusing (IEF) as a sample pre-concentrating and separation stage before presentation to an optical waveguide sensor. Current direct optical immunosensors are limited to fairly clean samples, as interference effects become very significant as the concentration of interferents increases. To overcome this problem, we intend to investigate IEF as a method by which the components of interest in a complex sample (such as blood) can be directed to specific locations on the surface of the sensor, while most, if not all, of the interferents are directed to other locations on the basis of their pIs. Since IEF is also a concentrating technique, the sensitivity of the assay should also be increased. The IEF separation channel will be fabricated on the surface of a Resonant Mirror optical sensor.

Summary

unavailable

Committee	Closed Committee - Engineering & Biological Systems (EBS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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