Award details

Monitoring of mutagenic and toxic chemicals by fluorescence induction and detection

Reference	E06511
Principal Investigator / Supervisor	Professor Richard Walmsley
Co-Investigators / Co-Supervisors	Professor Peter Fielden, Professor Nick Goddard
Institution	The University of Manchester
Department	Life Sciences
Funding type	Research
Value (£)	183,442
Status	Completed
Type	Research Grant
Start date	01/01/1997
End date	01/01/1999
Duration	24 months

Abstract

We propose to develop a monitoring system which responds to the presence of agents which induce stress responses in the budding yeast, Saccharomyces cerevisiae. The biological component of the system will exploit novel gene cassettes consisting of fusions between stress induced promoter sequences, and the gene encoding the readily detectable Green Fluorescent Protein. The detection system will exploit solid state light sources and detectors in conjunction with a multi channel flow injection system. Our experiments are designed to optimise conditions for the delivery of the yeast, the induction of the reporter and the detection of the fluorescence.

Summary

unavailable

Committee	Closed Committee - Engineering & Biological Systems (EBS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

I accept the terms and conditions of use (opens in new window)

export PDF file

back to list new search