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Enhancement of cytological gene expression assays in prokaryotes by development of enzyme-reporter quantification

Reference	E06333
Principal Investigator / Supervisor	Professor Michael Barer
Co-Investigators / Co-Supervisors
Institution	Newcastle University
Department	School of Cell and Molecular Biosciences
Funding type	Research
Value (£)	104,014
Status	Completed
Type	Research Grant
Start date	01/05/1996
End date	01/05/1998
Duration	24 months

Abstract

Recent demonstrations of the value and applications of a sensitive fluorogenic microscope-based assay of beta-galactosidase (lacZ) which detects activity at the single cell level has led the applicant to address current limitations to this approach at a generic level. The potential contribution of differences in substrate penetration to the site of hydrolysis will be approached by synthesis and evaluation of novel substrates which emit two distinct signals in proportion to local substrate and reaction product concentrations; distinction between recent and persisting reporter molecules will be improved by development of labile lacZ mutants; and the capacity of the microscope-based assay to analyse large populations of cells will be enhanced by image analysis software development.

Summary

unavailable

Committee	Closed Committee - Engineering & Biological Systems (EBS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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