BBSRC Portfolio Analyser
Award details
Design and synthesis of novel soluble cytochrome P450 mono-oxygenases for application in biocatalysis
Reference
E04280
Principal Investigator / Supervisor
Professor Sabine Flitsch
Co-Investigators /
Co-Supervisors
Professor Luet Wong
Institution
University of Edinburgh
Department
Sch of Chemistry
Funding type
Research
Value (£)
220,828
Status
Completed
Type
Research Grant
Start date
01/10/1995
End date
01/10/1998
Duration
36 months
Abstract
Mono-oxygenases can oxidise organic compounds at non-functionalised carbons and hence have great potential in biocatalysis. Progress has been slow because insufficient quantities of enzyme with suitable specificities have not been easily hitherto available. Cytochrome P450cam mono-oxygenase is a soluble enzyme which is easily accessible in recombinant form in large quantities but has narrow specificity. We propose to extend the substrate specificity of this mono-oxygenase. Thus a library of site-specific random mutants of P450cam will be generated and methods be developed to screen this mono-oxygenase library with substrates of interest.
Summary
unavailable
Committee
Closed Committee - Engineering & Biological Systems (EBS)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
X - not in an Initiative
Funding Scheme
X – not Funded via a specific Funding Scheme
I accept the
terms and conditions of use
(opens in new window)
export PDF file
back to list
new search