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The biophysics of macromolecular crowding - a comprehensive experimental study of model systems
Reference
E04113
Principal Investigator / Supervisor
Professor Wilson Poon
Co-Investigators /
Co-Supervisors
Professor Peter Pusey
Institution
University of Edinburgh
Department
Dept of Physics and Astronomy
Funding type
Research
Value (£)
190,086
Status
Completed
Type
Research Grant
Start date
01/01/1996
End date
31/03/1999
Duration
39 months
Abstract
Most in vivo biochemistry and in vitro biotechnology occurs in aqueous media with a high concentration of macromolecules or other suspended/solvated species (e.g. organelles). The resulting macromolecular crowding can have important consequences. Chemical equilibrium, and thus structural adoption, can be significantly altered. Diffusive processes can slow down by an order of magnitude. We propose an extensive experimental program to study phase behaviour and diffusion in well characterised model systems which mimic generic crowded biomedia. In particular, we will probe diffusion using a novel two-colour dynamic light scattering technique which allows us to study optically turbid, concentrated samples. Results obtained should provide a much-needed baseline for any future studies on more realistic systems.
Summary
unavailable
Committee
Closed Committee - Engineering & Biological Systems (EBS)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
X - not in an Initiative
Funding Scheme
X – not Funded via a specific Funding Scheme
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