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Role of DNA mismatch repair gene MLH1 in modulating effects of butyrate on gut epithelial cell kinetics and gene expression
Reference
D20173
Principal Investigator / Supervisor
Professor John Mathers
Co-Investigators /
Co-Supervisors
Dr Jonathan Coxhead
,
Dr E Williams
Institution
Newcastle University
Department
School of Medical Education Development
Funding type
Research
Value (£)
243,679
Status
Completed
Type
Research Grant
Start date
01/10/2003
End date
30/09/2006
Duration
36 months
Abstract
DNA mismatch repair (MMR) is one of 5 DNA repair systems required to maintain the integrity of the genome and has a key role in both hereditary and sporadic colorectal cancer. We have observed differential sensitivity of MMR deficient and proficient colonocytes to butyrate. We propose a series on in vivo and in vitro experiments to test the hypothesis that i) the MLH1 status of the gastrointestinal epithelial determines sensitivity to butyrate, and ii) that increased genomic instability/damage in response to butyrate triggers cell cycle arrest and/or apoptosis. The transcriptional events mediating these effects will be investigated using microarray technology.
Summary
unavailable
Committee
Closed Committee - Agri-food (AF)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
X - not in an Initiative
Funding Scheme
X – not Funded via a specific Funding Scheme
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