BBSRC Portfolio Analyser
Award details
Apoplastic hydroxyl radicals and their in-vivo action on textural polysaccharides in ripening fruit tissues
Reference
D19626
Principal Investigator / Supervisor
Professor Stephen Fry
Co-Investigators /
Co-Supervisors
Institution
University of Edinburgh
Department
Inst for Molecular Plant Science
Funding type
Research
Value (£)
253,380
Status
Completed
Type
Research Grant
Start date
01/12/2003
End date
30/11/2006
Duration
36 months
Abstract
The hydroxyl radical (OH) non-enzymically cleaves polysaccharides in vitro. We will test whether .OH is formed in the fruit apoplast in vivo and attacks structural polysaccharides, contributing to fruit softening. We will use pears (because of initial success) and several other fruits to look for OH by two novel techniques: (a) chemical fingerprinting of OH-attacked polysaccharides in fruit; (b) detection of apoplastic OH by a membrane-impermeant reporter molecule that reacts specifically with OH to yield 3H20. The 2 methods will be further refined, and OH-scavengers used as a test of validity. If apoplastic OH is widespread, this will be of great importance in re-defining future opportunities for commercial control of fruit quality.
Summary
unavailable
Committee
Closed Committee - Agri-food (AF)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
X - not in an Initiative
Funding Scheme
X – not Funded via a specific Funding Scheme
I accept the
terms and conditions of use
(opens in new window)
export PDF file
back to list
new search