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Roles of electron transport through nitrate and nitrite reductases in survival growth and colonisation of Campylobacter jejuni
Reference
D18189
Principal Investigator / Supervisor
Professor Dave Kelly
Co-Investigators /
Co-Supervisors
Institution
University of Sheffield
Department
Molecular Biology and Biotechnology
Funding type
Research
Value (£)
214,968
Status
Completed
Type
Research Grant
Start date
01/08/2003
End date
31/07/2006
Duration
36 months
Abstract
We have recently shown that Campylobacter jejuni can use energy conserving electron transport pathways to a variety of alternative electron acceptors to support growth under oxygen-limited conditions. C. jejuni possesses periplasmic nitrate and nitrite reductases which may play several roles in survival in the environment and in vivo. We propose to (i) determine the function of the genes in the Nap and Nrf operons in enzyme biogenesis and electron transport to nitrate and nitrite (ii) evaluate the role of Nap and Nrf in vivo (iii) test the hypothesis that Nrf can also act as an nitric oxide reductase and thus contribute to NO detoxification (iv) determine the consequences for nitrite and NO reduction of the unique active site haem ligation of the C. jejuni Nrf, by spectroscopic characterisation of purified wild-type and mutant enzymes.
Summary
unavailable
Committee
Closed Committee - Agri-food (AF)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
X - not in an Initiative
Funding Scheme
X – not Funded via a specific Funding Scheme
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