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A multidisciplinary study of Salmonella-protozoa interactions on surfaces liquid culture and soil: bacterial phenotype dynamics

Reference	D18179
Principal Investigator / Supervisor	Professor Elizabeth Wellington
Co-Investigators / Co-Supervisors	Professor Nigel Burroughs, Professor William Gaze
Institution	University of Warwick
Department	Biological Sciences
Funding type	Research
Value (£)	307,928
Status	Completed
Type	Research Grant
Start date	15/02/2003
End date	14/01/2007
Duration	47 months

Abstract

The aims of this project are to elucidate protozoa-salmonellae interactions on surfaces, in aquatic systems and in soils. Sophisticated image analysis techniques and a Fluorescence-Activated Cell Sorter (FACS) will be employed. Mathematical modelling and data analysis will be used to study interaction dynamics, allowing comparison of mechanisms and rates. Salmonellae populations will be examined using a gfp gene reporter, live-dead, and cell proliferation and cell cycle analysis. Phenotypic change of salmonellae on passage through protozoa will be studied, particularly induction of resistance to antibiotics, filamentous growth and resuscitation of viable but unculturable cells. Methods used to detect salmonellae in the environment will include immunomagnetic capture, Nycodenz extraction from soil and protozan culture to detect intracellular growth.

Summary

unavailable

Committee	Closed Committee - Agri-food (AF)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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