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Role of lymphostatin in the colonisation of cattle by enterohaemorragic Escherichia coli

ReferenceD17455
Principal Investigator / Supervisor Professor Mark Stevens
Co-Investigators /
Co-Supervisors
Institution The Pirbright Institute
DepartmentDiv of Microbiology Compton
Funding typeResearch
Value (£) 283,708
StatusCompleted
TypeResearch Grant
Start date 21/10/2002
End date 21/10/2005
Duration36 months

Abstract

Enterohaemorrhagic E. coli (EHEC) are emerging zoonotic pathogens and cattle are a major reservoir of human infections. We have identified a gene (lifA) that influences intestinal colonisation of calves by non-0157 EHEC. LifA (lymphostatin) acts both as an inhibitor of lymphocyte function and as an adhesin in vitro. We aim to determine how lymphostatin influences intestinal colonisation through integration of genetic techniques and the in vitro and in vivo analysis of EHEC-interactions with bovine epithelial and lymphoid cells. The roles of an E. coli O157:H7 lifA homologue (17095) and truncated lifA gene will also be investigated. We will dissect the functional domains of lymphostatin to gain insights into its mechanism of action.

Summary

unavailable
Committee Closed Committee - Agri-food (AF)
Research TopicsX – not assigned to a current Research Topic
Research PriorityX – Research Priority information not available
Research Initiative X - not in an Initiative
Funding SchemeX – not Funded via a specific Funding Scheme
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