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Nutrient-gene interactions underlying individual response of selenoenzyme gene expression to dietary selenium

Reference	D15232
Principal Investigator / Supervisor	Professor John Hesketh
Co-Investigators / Co-Supervisors	Professor John Mathers
Institution	Newcastle University
Department	Agriculture Food and Rural Development
Funding type	Research
Value (£)	328,720
Status	Completed
Type	Research Grant
Start date	01/02/2002
End date	01/02/2005
Duration	36 months

Abstract

The dietary intake of the micronutrient Se by the UK population is marginal for optimal health. Se is incorporated as the amino-acid selenocysteine into selenoproteins during translation; this requires a structure in the 3 prime untranslated region (3 prime UTR) of the mRNA. The project will investigate the functional significance of a polymorphism in the gene sequence of the phospholipid hydroperoxide glutathione peroxidase 3 prime UTR and define the role of 3 prime UTR-protein interactions in the prioritisation of Se when Se supply is marginal. The effect of the genetic polymorphism will be analysed using reporter gene expression in transfected cells. Footprinting and UV crosslinking methods will be used to describe the effects of Se supply on RNA-protein binding. The influence of genotype on dietary Se requirements will be studied in a nutritional intervention study in human volunteers.

Summary

unavailable

Committee	Closed Committee - Agri-food (AF)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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