BBSRC Portfolio Analyser
Award details
Generation of enhancer trap lines of rice (Oryza sativa L.) expressing gal4 and gfp in specific cell types
Reference
D15077
Principal Investigator / Supervisor
Professor Julian Hibberd
Co-Investigators /
Co-Supervisors
Institution
University of Cambridge
Department
Plant Sciences
Funding type
Research
Value (£)
216,844
Status
Completed
Type
Research Grant
Start date
01/09/2001
End date
31/08/2004
Duration
36 months
Abstract
The aim is to generate a set of rice lines expressing an exogenous transcription factor (ga14) in specific cells or tissues. This will provide for the first time a powerful tool for the targeted expression of transgenes in an important monocotyledonous crop. ga14 will be introduced with gfp, a gene encoding a fluorescent protein, which will enable visualisation of expression patterns. This will be done semi-randomly using supervirulent Agrobacterium tumefaciens. Cell-specific patterns of ga14/gfp expression (determined by the site of gene insertion) will be documented electronically. The lines will then be made available to the broader scientific community. We also aim to trial cell-specific expression of transgenes using these lines.
Summary
unavailable
Committee
Closed Committee - Agri-food (AF)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
X - not in an Initiative
Funding Scheme
X – not Funded via a specific Funding Scheme
I accept the
terms and conditions of use
(opens in new window)
export PDF file
back to list
new search