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Dietary fatty acids effects on gene transcription in normal human epithelial cells

Reference	D14509
Principal Investigator / Supervisor	Professor Jennifer Southgate
Co-Investigators / Co-Supervisors	Dr Ludwik Trejdosiewicz
Institution	University of York
Department	Biology
Funding type	Research
Value (£)	260,100
Status	Completed
Type	Research Grant
Start date	01/06/2001
End date	31/10/2004
Duration	41 months

Abstract

We have been using a normal human uro-epithelial (NHU) cell system to investigate the effects of physiologically-presented dietary long chain fatty acids (FA) on proliferation and gene expression. As a consequence of our work to date, we propose to a) identify fatty acid metabolites produced in NHU cells using HPLC and mass spectroscopy, b) use inhibitors and specific metabolites to determine which species are responsible for the anti-proliferative and gene expression changes in NHU cells, c) determine whether the FA act through peroxisome proliferator activated receptors and d) microarray and screen cDNA libraries representing genes differentially expressed in response to LA.

Summary

unavailable

Committee	Closed Committee - Agri-food (AF)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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