Award details

Development of wheat allele specific oligonucleotides (ASOs) from existing microsatellite markers and their use in developing multi-locus, multi-allele genotyping assays

Reference	D14009
Principal Investigator / Supervisor	Dr Mike Gale
Co-Investigators / Co-Supervisors	Professor Keith Edwards, Dr Jiahui Zhu
Institution	John Innes Centre
Department	John Innes Centre Department
Funding type	Research
Value (£)	230,352
Status	Completed
Type	Research Grant
Start date	04/04/2001
End date	04/04/2004
Duration	36 months

Abstract

Wheat is the most important cereal grown in the UK. Unfortunately, in terms of molecular breeding, it is also the most difficult. Current wheat molecular markers such as RFLPs, AFLPs and SSRs, are limited to gel-based technologies. In this proposal we intend to utilise the highly polymorphic flanking regions of existing microsatellite markers to develop allele specific oligonucleotides. In turn these ASOs will be exploited in the development of a DNA genotyping chip and MALDI-TOF analysis to characterise wheat genotypes or varieties. The sequences developed by this programme will be of considerable academic and commercial interest.

Summary

unavailable

Committee	Closed Committee - Agri-food (AF)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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