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The role of plastid-located lipoxygenase in aroma volatile production in tomato fruit

ReferenceD14006
Principal Investigator / Supervisor Professor Donald Grierson
Co-Investigators /
Co-Supervisors
Institution University of Nottingham
DepartmentSch of Biosciences
Funding typeResearch
Value (£) 223,396
StatusCompleted
TypeResearch Grant
Start date 01/01/2001
End date 06/03/2004
Duration38 months

Abstract

The aim of the research programme is to critically evaluate the role of TomloxC, a plastid-located tomato lipoxygenase, in fruit aroma volatile production. We have previously demonstrated that aroma volatile production was unaffected by antisense inhibition of two of the three lipoxygenase genes expressed in tomato fruit. This suggests that the third lipoxygenase, TomloxC is specifically responsible for production of the major tomato fruit aroma compounds hexenal and hexanal. TomloxC will be over-expressed or down-regulated in both transgenic wild type fruit and those of a ripening mutant (rin) with poor flavour/aroma, and the effect of aroma volatiles determined. Substrate specificity of TomloxC, and the identity of reaction products will be investigated following expression of TomloxC in E. coli.

Summary

unavailable
Committee Closed Committee - Agri-food (AF)
Research TopicsX – not assigned to a current Research Topic
Research PriorityX – Research Priority information not available
Research Initiative X - not in an Initiative
Funding SchemeX – not Funded via a specific Funding Scheme
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