Award details

Development of a universal DNA microarray to investigate genome diversity in Campylobacter jejuni

Reference	D13925
Principal Investigator / Supervisor	Professor Brendan Wren
Co-Investigators / Co-Supervisors
Institution	London Sch of Hygiene and Trop Medicine
Department	Infectious and Tropical Diseases
Funding type	Research
Value (£)	210,856
Status	Completed
Type	Research Grant
Start date	01/10/2000
End date	01/10/2003
Duration	36 months

Abstract

C. jejuni is the major cause of food-borne gastrointestinal disease and is an acknowledged clinical and economic problem. C. jejuni causes a wide spectrum of disease which is partly reflected by strain heterogeneity. The genetic basis for C. jejuni diversity is poorly understood. Recently the applicant has constructed a DNA microarray containing all 1654 genes from C. jejuni NCTC11168. Using a combination of whole-genome long range PCR and subtractive hybridisation, additional genes from 90 isolates of diverse origins will be characterised and added to the NCTC11168 microarray. The new universal DNA microarray will be used to assess the genome diversity of over 300 C. jejuni strains of known origin and phenotype. These studies will provide novel insights into the pathogenicity, molecular epidemiology and evolution of virulence of the organism and should lead to rationale intervention strategies to eliminate C. jejuni from the food chain.

Summary

unavailable

Committee	Closed Committee - Agri-food (AF)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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