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In vitro studies of the interaction between enterohaemorrhagic Escherichia coli and human intestinal mucosa

Reference	D13600
Principal Investigator / Supervisor	Dr Alan Phillips
Co-Investigators / Co-Supervisors	Professor Gad Frankel
Institution	University College London
Department	Department of Paediatric Gasroenterology
Funding type	Research
Value (£)	154,800
Status	Completed
Type	Research Grant
Start date	03/04/2001
End date	03/04/2004
Duration	36 months

Abstract

Enterohaemorrhagic E. coli [EHEC] are a major risk to health yet the lack of a reproducible model system has slowed progress in studying their interaction with human intestine. We have established in vitro organ culture [IVOC] of human intestinal mucosa with EHEC O157:H7 in which attaching-effacing lesion formation is evident. Colonisation is targeted to follicle associated epithelium, a previously unrecognised phenomenon in EHEC. This system will allow us to: 1) study initial colonisation events in various EHEC strains in order to determine the pattern of tissue tropism and the factors involved; 2) assess Shiga toxin involvement during tissue colonisation, and 3) detect unappreciated virulence factors using in vivo expression technology.

Summary

unavailable

Committee	Closed Committee - Agri-food (AF)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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