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Generation of normalised region-specific cDNA libraries in domestic farmed species: tools for functional genome analysis

Reference	D13572
Principal Investigator / Supervisor	Professor Darren Griffin
Co-Investigators / Co-Supervisors	Professor Alan Archibald, Professor David Burt, Mr Julio Masabanda
Institution	Brunel University London
Department	Biological Sciences
Funding type	Research
Value (£)	137,653
Status	Completed
Type	Research Grant
Start date	01/11/2000
End date	01/11/2002
Duration	24 months

Abstract

Conventional cDNA libraries are invaluable for genome research as they provide access to functional sequences in a compact format. Nevertheless inefficiencies in their isolation are known e.g. the representation of sequences is proportional to the degree of transcription in the tissue of origin. Thus rarely expressed sequences are underrepresented and this is only partially circumvented by normalisation. Knowledge of map location is critical to narrowing the search for a gene however sequencing the entire genome or even millions of ESTs is not currently an option for farm animals. Thus strategies for focusing on regions of interest are essential. We therefore propose to combine the strengths of a cDNA strategy with an approach that includes map information to generate and evaluate resources (i.e. region specific normalised cDNA libraries) for gene discovery in farm animals.

Summary

unavailable

Committee	Closed Committee - Agri-food (AF)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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