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High-throughput whole-genome PCR with MADGE and SP-PCR for the analysis of genomic diversity in the enteric bacteria

ReferenceD13414
Principal Investigator / Supervisor Professor Mark Pallen
Co-Investigators /
Co-Supervisors
Institution University of Birmingham
DepartmentMedical Sciences - Medicine
Funding typeResearch
Value (£) 158,806
StatusCompleted
TypeResearch Grant
Start date 14/11/2000
End date 13/02/2004
Duration39 months

Abstract

The enteric bacteria (family Enterobacteriaceae) are a remarkably versatile range of species that includes important pathogens of humans, animals and plants, two of which (Escherichia coli and Salmonella enterica) commonly contaminate the human food chain. There are pressing scientific and practical needs to develop methods that allow rapid, efficient and user- friendly assessment and analysis of genomic diversity within the enteric bacteria. Building on recent technical advances within our own laboratories, we aim to develop a novel high-throughput approach (whole-genome- PCR with microplate array diagonal gel electrophoresis) for assessing genomic diversity and for detecting large-scale chromosomal insertions or deletions that will be robust, simple and cheap enough for use in routine molecular epidemiology, but that will also link epidemiological findings to the genomic determinants of pathogenicity and provide a discovery platform for identifying new pathogenicity islands and prophages linked to virulence. (Joint with grant 51/D13422)

Summary

unavailable
Committee Closed Committee - Agri-food (AF)
Research TopicsX – not assigned to a current Research Topic
Research PriorityX – Research Priority information not available
Research Initiative X - not in an Initiative
Funding SchemeX – not Funded via a specific Funding Scheme
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