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A transgenic approach to analyse susceptibility to Pre-Harvest Sprouting and potential for improved resistance in wheat
Reference
D13143
Principal Investigator / Supervisor
Professor Michael Holdsworth
Co-Investigators /
Co-Supervisors
Dr John Lenton
Institution
Rothamsted Research
Department
Plant Pathology and Microbiology
Funding type
Research
Value (£)
103,824
Status
Completed
Type
Research Grant
Start date
02/10/2000
End date
02/10/2002
Duration
24 months
Abstract
Pre-Harvest Sprouting (PHS) is an important agronomic trait in wheat, contributing to the quality and uniformity of breadmaking flour. We wish to test a hypothesis that expression of the wild oat transcription factor AfVP1 in transgenic wheat plants will alter gene expression programmes in the embryo and aleurone, increasing dormancy of the seeds, and thus reducing susceptibility to PHS. We will analyse the expression of wheat alpha-amylase enzymes (that cause spoilage of flour in sprouted lots) at the levels of RNA and protein expression, and activity in seed extracts as the Hagberg Falling Number test to determine whether the expression of these enzymes is altered in transgenic seeds.
Summary
unavailable
Committee
Closed Committee - Agri-food (AF)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
X - not in an Initiative
Funding Scheme
X – not Funded via a specific Funding Scheme
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