Award details

A simple system for protein stacking (pyramiding) in GM crops

Reference	D13022
Principal Investigator / Supervisor	Professor Claire Halpin
Co-Investigators / Co-Supervisors	Professor Martin Ryan
Institution	University of Dundee
Department	College of Life Sciences
Funding type	Research
Value (£)	138,668
Status	Completed
Type	Research Grant
Start date	01/09/2000
End date	01/09/2003
Duration	36 months

Abstract

A possibility of introducing multiple heterologous proteins, perhaps entire biochemical pathways into plants is an exciting prospect. We have developed a novel method for co- expressing multiple proteins in plants by encoding them in artificial self- dissociating polyprotein. A short peptide (2A) of unique function from foot-and-mouth disease virus, separates distinct proteins within the polyprotein and effects its co- translational dissociation into discrete protein products. Commercial uptake of this technology is prevented by concerns over consumer resistance to the use of pathogenic animal virus sequences. The proposal attacks this problem by developing a 'plant' or 'artificial' 2A homologue that will be removed during protein maturation. Most importantly, this work will yield invaluable insights into how this novel 2A peptide works.

Summary

unavailable

Committee	Closed Committee - Agri-food (AF)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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