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Expression and release of toxin and converting phage in Shiga toxin-producing Escherichia coli (STEC)

Reference	D12994
Principal Investigator / Supervisor	Professor Michael Barer
Co-Investigators / Co-Supervisors	Professor Colin Harwood, Mr Patrick Kimmitt
Institution	University of Leicester
Department	Microbiology & Immunology
Funding type	Research
Value (£)	190,252
Status	Completed
Type	Research Grant
Start date	01/04/2000
End date	01/04/2003
Duration	36 months

Abstract

Shiga toxins (Stx) enable STECs to cause diarrhoea and kidney damage. Results in this and other labs show that Stx can be expressed as late products of converting phage and that both are induced via the SOS response. Here, we shall investigate 1) whether Stx2 can be expressed independently from the SOS response and/or phage 2) the central paradox that while Stx synthesis appears linked to phage-mediated lysis, both StxA and B genes encode signal sequences. Our reporter constructs, ability to monitor near real time expression at the single cell level and experience with prokaryotic secretion studies provide an excellent platform for these studies.

Summary

unavailable

Committee	Closed Committee - Agri-food (AF)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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