Award details

The genetic basis of sex determination in fish: a molecular-cytogenetic analysis

Reference	D11975
Principal Investigator / Supervisor	Dr David Penman
Co-Investigators / Co-Supervisors	Dr N Bromage, Professor Darren Griffin
Institution	University of Stirling
Department	Institute of Aquaculture
Funding type	Research
Value (£)	203,732
Status	Completed
Type	Research Grant
Start date	17/04/2000
End date	17/10/2003
Duration	42 months

Abstract

Control over sex ratios is important in the culture of many species of fish. While this has been achieved in several fish, it is hindered by the current inability in most cultured species to identify sex chromosomes and a lack of sex-linked markers. We have identified the putative sex chromosomes in a model (but also commercially important) fish, the Nile tilapia (Oreochromis niloticus), by synaptonemal complex analysis. In this proposal we aim to utilise the microdissection, DOP-PCR amplification, FISH cloning and characterisation of DNA sequences from the target chromosomal area to develop a methodology with potential application for the identification and analysis of sex chromosomes in cultured fish species. Preliminary data has shown that probes specific to part of chromosome 1 (the putative sex chromosome) can be generated by microdissection and PCR of parts of this chromosome from metaphase spreads prepared from primary lymphocyte cultures.

Summary

unavailable

Committee	Closed Committee - Agri-food (AF)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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