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The synthesis of plant cell walls: the role of the Golgi GDP-mannose sugar nucleotide transporter
Reference
D11626
Principal Investigator / Supervisor
Professor Paul Dupree
Co-Investigators /
Co-Supervisors
Institution
University of Cambridge
Department
Biochemistry
Funding type
Research
Value (£)
214,928
Status
Completed
Type
Research Grant
Start date
01/10/1999
End date
01/10/2002
Duration
36 months
Abstract
The feasibility of manipulating cell wall polysaccharide synthesis through alteration of Golgi sugar nucleotide transporter activity will be demonstrated. We will confirm our identification of a gene from Arabidopsis encoding a protein that translocates GDP-mannose into the Golgi lumen, using yeast complementation, and localisation of the protein in plants by microscopy. Next, we will demonstrate the role of the transporter in GDP-mannose transport, by use of mutant plants and of a transport assay with isolated plant Golgi vesicles. Finally, we will investigate the effect on polysaccharide synthesis of the manipulation of Golgi luminal GDP- mannose availability through selection and generation of transporter mutants and over- expressing plants. This work will thus provide enabling technology for the commercial generation or selection of plants with altered cell wall polysaccharides.
Summary
unavailable
Committee
Closed Committee - Agri-food (AF)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
X - not in an Initiative
Funding Scheme
X – not Funded via a specific Funding Scheme
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