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The roles of plastidial starch-modifying enzymes in determining starch quantity and quality in plants

ReferenceD11089
Principal Investigator / Supervisor Dr S Smith
Co-Investigators /
Co-Supervisors
Institution University of Edinburgh
DepartmentInst of Cell and Molecular Biology
Funding typeResearch
Value (£) 169,764
StatusCompleted
TypeResearch Grant
Start date 01/06/1999
End date 01/06/2002
Duration36 months

Abstract

Starch-modifying enzymes are present in plastids during starch synthesis as well as during breakdown. They may thus play a key role in the determination of starch quality and quantity in plants. However, the precise functions of each of these enzymes during starch synthesis and during breakdown, are unknown. Our aim is to determine the function of each enzyme by studying starch structure and metabolism in Arabidopsis mutants lacking specific plastidial isoforms of debranching and disproportionating enzymes, phosphorylase, amylase and alpha-glucosidase. We have already identified three mutants lacking debranching enzyme, endoamylase and disproportionating enzyme, and will isolate others using a reverse- genetics approach. Our studies will clarify unresolved issues such as the role of debranching enzymes in starch synthesis, and the impact of turnover on starch yield. (Joint with grant 208/D11090)

Summary

unavailable
Committee Closed Committee - Agri-food (AF)
Research TopicsX – not assigned to a current Research Topic
Research PriorityX – Research Priority information not available
Research Initiative X - not in an Initiative
Funding SchemeX – not Funded via a specific Funding Scheme
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