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Award details
Use of a two component selection system to control transgene inheritance in field grown crop plants
Reference
D10349
Principal Investigator / Supervisor
Professor Rupert Fray
Co-Investigators /
Co-Supervisors
Institution
University of Nottingham
Department
Sch of Biosciences
Funding type
Research
Value (£)
143,983
Status
Completed
Type
Research Grant
Start date
01/02/1999
End date
01/02/2002
Duration
36 months
Abstract
Funding is requested for the development of a novel system for the prevention, without destroying crop fertility, of the environmental escape of transgenes from crop plants. The system will utilise a bacterial Type IIs restriction/modification system, with the sequence specific methylase transgene being incorporated into the chloroplast genome and the corresponding endonuclease transgene forming part of the conventional nuclear located T-DNA complex. The endonuclease will be targeted to the chloroplast via a chloroplast targeting sequence translationally fused at the N- terminus. Expression of this endonuclease will be lethal other than in engineered cytoplasmic background. The system also provides an in field selection against loss of chloroplast located transgenes.
Summary
unavailable
Committee
Closed Committee - Agri-food (AF)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
X - not in an Initiative
Funding Scheme
X – not Funded via a specific Funding Scheme
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