Award details

Characterisation of the interaction of enterohaemorrhagic Escherichia coli with intestinal mucosae

Reference	D10262
Principal Investigator / Supervisor	Professor Gad Frankel
Co-Investigators / Co-Supervisors	Dr Alan Phillips
Institution	Imperial College London
Department	Biological Sciences
Funding type	Research
Value (£)	154,314
Status	Completed
Type	Research Grant
Start date	01/10/1998
End date	01/10/2001
Duration	36 months

Abstract

Enterohaemorrhagic E. coli [EHEC] infections are an increasingly important zoonosis. Putative virulence factors have been identified, but their role in pathogenesis and host specificity remains unclear. We aim to study the interaction of wild type and defined mutant strains of EHEC with intestinal mucosae from humans and cattle, natural host species. Early host/pathogen interactions will be assessed in primary intestinal biopsies using in vitro organ culture. Subsequent events in pathogenesis will be studied using the ligated intestinal loop model in cattle which allows quantification of intestinal invasion, secretory and inflammatory responses. Translocation of specific virulence factors will be assessed by immunofluorescent and confocal microscopy. Signature tagged mutagenesis will be exploited in vivo to identify novel virulence genes. (Joint with grant D10261).

Summary

unavailable

Committee	Closed Committee - Agri-food (AF)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

Associated awards:

D10261 Characterisation of the interaction of enterohaemorrhagic Escherichia coli with intestinal mucosae

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