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Manipulating the phenolic acid content and digestibility of forage grass cell-walls by targeted expression of a ferulic acid esterase gene

Reference	D10116
Principal Investigator / Supervisor	Professor Phillip Morris
Co-Investigators / Co-Supervisors	Professor Michael Theodorou, Professor Howard Thomas
Institution	Inst of Grassland and Environmental Res
Department	Cell Biology Department
Funding type	Research
Value (£)	165,808
Status	Completed
Type	Research Grant
Start date	01/03/1999
End date	01/03/2002
Duration	36 months

Abstract

We propose to test novel strategies and develop 'proof of principle' for controlling the level of phenolic acids in grass cell walls and their crosslinking to cell-wall polysaccharides to increase our understanding of their role in moderating rates of cell wall digestion and carbohydrate availability in the rumen. Strategies based on modification of the levels of phenolic acids both in pre-existing cell walls and incorporation into cell wall precursors by targeted and inducible expression of a fungal ferulic acid esterases gene will be tested. The efficiency of the gene targeting/induction strategies and the effects of these modifications on cell wall composition, plant development and the rate of cell wall digestion under rumen-like conditions will be tested in transformed cell suspension cultures and in regenerated transgenic plants.

Summary

unavailable

Committee	Closed Committee - Agri-food (AF)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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