BBSRC Portfolio Analyser
Award details
Development of marker-free ('clean gene') transgenic crop plants using agrobacterium-based clean gene technology
Reference
D10076
Principal Investigator / Supervisor
Prof. Phillip Mullineaux
Co-Investigators /
Co-Supervisors
Institution
John Innes Centre
Department
Metabolic Biology
Funding type
Research
Value (£)
235,632
Status
Completed
Type
Research Grant
Start date
01/10/1998
End date
01/10/2001
Duration
36 months
Abstract
The 'clean gene' technology to be developed in this project uses existing Agrobacterium-mediated procedures and employs a newly developed highly flexible binary Ti vector system which has been funded by the BBSRC. This approach relies on the co-transformation into plants of two T-DNAs, only one of which contains the selectable marker gene. In a proportion of transformants, the integration of the T- DNAs into separate loci will occur. Subsequently, a self-crossing of the transformant will lead to segregation of the marker T-DNA from a T-DNA carrying a test gene. The project will determine the best parameters for achieving the maximum frequency of segregation of the T-DNAs, provide a flexible plasmid system to encourage use, techniques to eliminate primary transformants with linked T-DNAs prior to transfer to containment glasshouse and a demonstration of its use in two UK crops.
Summary
unavailable
Committee
Closed Committee - Agri-food (AF)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
X - not in an Initiative
Funding Scheme
X – not Funded via a specific Funding Scheme
I accept the
terms and conditions of use
(opens in new window)
export PDF file
back to list
new search