Award details

Development of the transposable element mariner as a vector for efficient production of transgenic chickens

Reference	D10065
Principal Investigator / Supervisor	Professor Helen Sang
Co-Investigators / Co-Supervisors	Professor David Finnegan
Institution	The Roslin Institute
Department	Gene Expression and Development
Funding type	Research
Value (£)	320,827
Status	Completed
Type	Research Grant
Start date	01/10/1998
End date	11/06/2002
Duration	44 months

Abstract

We have developed a method for production of transgenic chickens, involving microinjection of the chick zygote followed by embryo culture. We aim to improve the frequency of stable transgene incorporation and have preliminary results indicating that the transposable element marineris a good candidate for development of a vector system. Our objectives are to demonstrate that transgene constructs can be introduced into the chicken germline, using a mariner-derived vector, and that the transgenes are expressed in the predicted tissue-specific manner. We also aim to develop the vector system by testing different methods of introducing the transposase function, necessary for vector integration, and to determine the stability of integrated copies of mariner when transmitted through the germline of transgenic birds.

Summary

unavailable

Committee	Closed Committee - Agri-food (AF)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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