Award details

New tools for cereal genome manipulation; enabling technologies for plant artificial chromosomes

Reference	D09456
Principal Investigator / Supervisor	Professor Phillip Morris
Co-Investigators / Co-Supervisors	Professor Keith Edwards, Professor Robert Jones, Dr Tim Langdon
Institution	Inst of Grassland and Environmental Res
Department	Cell Biology Department
Funding type	Research
Value (£)	131,003
Status	Completed
Type	Research Grant
Start date	01/03/1999
End date	13/02/2002
Duration	35 months

Abstract

Chromosomal vectors offer solutions to a number of pressing problems in the creation of transgenic grass crops. Construction of such vectors has become feasible following the recent identification of ubiquitous cereal centromere components and developments in human artificial chromosome assembly in vivo. Our collaboration brings together the necessary skills and resources to begin a construction programme developing universal chromosomal vectors for grasses and cereals. Our first priority is to refine methods to deliver existing components. We will apply microinjection and liposome fusion protocols to develop intracellular competition assays for replication and segregation by maize yeast artificial chromosome clones. We will also develop assays for integration competence of virus-like particles as a prerequisite for developing targeted fragmentation strategies.

Summary

unavailable

Committee	Closed Committee - Agri-food (AF)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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