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The role of the plastidial pyruvate transporter in control of carbon flux to storage oil in oilseed rape

Reference	D09342
Principal Investigator / Supervisor	Dr Stephen Rawsthorne
Co-Investigators / Co-Supervisors
Institution	John Innes Centre
Department	John Innes Centre Department
Funding type	Research
Value (£)	214,812
Status	Completed
Type	Research Grant
Start date	13/07/1998
End date	13/07/2001
Duration	36 months

Abstract

We will determine the flux control coefficient of the plastidial pyruvate transporter (PPT) for oil synthesis in developing rapeseed (Brassica napus) in vivo. A full-length cDNA encoding the B. napus PPT will be isolated. Over and under expression of the PPT will be achieved in transgenic oilseed rape expressing sense or antisense constructs containing the cDNA driven by the seed-specific oleosin promoter. The activity of the PPT, and the rates of plastidial fatty acid synthesis and oil synthesis will be measured during embryo development in transgenic lines at stages which we have identified as important in the timing of PPT activity in control lines. We will also determine how manipulation of seed oil content in this crop species affects partitioning of carbon to other seed storage products.

Summary

unavailable

Committee	Closed Committee - Agri-food (AF)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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