Award details

Coordinate regulation of intestinal amino acid and peptide transporters

Reference	D09247
Principal Investigator / Supervisor	Professor Barry Hirst
Co-Investigators / Co-Supervisors
Institution	Newcastle University
Department	School of Cell and Molecular Biosciences
Funding type	Research
Value (£)	271,596
Status	Completed
Type	Research Grant
Start date	01/02/1998
End date	30/09/2001
Duration	44 months

Abstract

Regulation of nutrients of gastrointestinal gene expression is important in the response of the gut to diet. An integral understanding of adaptation to diet requires the study of coordinated gene responses. The aim of this project is to investigate the hypothesis that the adaptive response to dietary protein levels involves the coordinated regulation of peptide and amino acid transporters, by elucidating patterns of coordinate regulation of gastrointestinal amino acid and peptide transporters, at the molecular level, in response to total dietary nitrogen levels and to specific amino acids. Levels of transporter expression in human intestinal Caco-2 cells will be quantified by RT-PCR, immunohistochemistry and western blotting. The functional consequences of regulation will be established by studies on the cellular uptake and transport of radiolabelled substrates. Comparative studies in the rate will place the findings in a physiological context. The results will be relevant to a number of dietary health issues.

Summary

unavailable

Committee	Closed Committee - Agri-food (AF)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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