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The role of apolipoprotein B-mRNA editing in lipid absorption and chylomicron assembly in apobec-1 knock-out mice compared with wild type mice

Reference	D09212
Principal Investigator / Supervisor	Dr J Higgins
Co-Investigators / Co-Supervisors
Institution	University of Sheffield
Department	Molecular Biology and Biotechnology
Funding type	Research
Value (£)	203,176
Status	Completed
Type	Research Grant
Start date	06/04/1998
End date	11/12/2001
Duration	44 months

Abstract

Apo-B48, the essential protein for absorption of lipids and assembly of chylomicrons in all mammals including man, is a truncated form of apo-B100, produced through post- transcriptional editing of the apo-B100 mRNA. We have available a mouse line in which the gene (apo-bec-1) for the editing enzyme has been deleted. The intestine of apo-bec-1 knock-out mice produces only apo-B100 instead of apo-B48. Comparison of the wild type and knock-out mice provides a unique model for determining the relevance of apo-B mRNA editing in lipid absorption in man. We will use this model to investigate the molecular events in the assembly of chylomicrons and investigate how this process is modified by altering the amounts and composition of dietary fat.

Summary

unavailable

Committee	Closed Committee - Agri-food (AF)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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