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Co-operative activity of H+/solute symport and Na+/H+ exchange at the intestinal epithelial brush-border membrane
Reference
D09145
Principal Investigator / Supervisor
Professor David Thwaites
Co-Investigators /
Co-Supervisors
Institution
Newcastle University
Department
School of Cell and Molecular Biosciences
Funding type
Research
Value (£)
212,823
Status
Completed
Type
Research Grant
Start date
01/08/1998
End date
28/02/2002
Duration
43 months
Abstract
This project aims to improve the fundamental understanding of how epithelial function (vectorial transport of solute) is optimised by cooperative activity of individual membrane transporters identified at the molecular level. Experiments using the cultured human intestinal epithelial cell line Caco-2 will determine the capacity of Na+/H+ exchange activity and H+solute transport at apical and basal cell membranes by microspectrofluorimetry and radioisotopic measurements. Localised areas of acidic pHi will be measured directly by confocal-laser scanning microscopy of SNAFL-calcein loaded cells. Experiments in epithelial sheets isolated from rat and rabbit intestine will determine the generality of the proposed model.
Summary
unavailable
Committee
Closed Committee - Agri-food (AF)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
X - not in an Initiative
Funding Scheme
X – not Funded via a specific Funding Scheme
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