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Developing informative microsatellites for marker-assisted breeding in brassica crops
Reference
D08078
Principal Investigator / Supervisor
Professor Keith Edwards
Co-Investigators /
Co-Supervisors
Dr Martin Trick
Institution
Rothamsted Research
Department
Rothamsted Research Department
Funding type
Research
Value (£)
185,251
Status
Completed
Type
Research Grant
Start date
05/01/1998
End date
05/12/2001
Duration
47 months
Abstract
Marker-assisted breeding can accelerate the introduction of beneficial traits into crop plants. Existing Brassica RFLP technology allows marker-aided selection, but large- scale application is hampered by limited intervarietal polymorphism and difficulties in automation. A set of 300 highly polymorphic Brassica microsatellites will be developed to complement existing markers. This will involve: (1) generating gridded genomic libraries enriched for microsatellites; (2) screening with oligonucleotides and PCR-based assays to pre-select useful clones; (3) DNA sequencing and primer design; (4) evaluating polymorphism in Brassica material; (5) mapping the microsatellite loci in Brassica napus and (6) testing the new markers in a range of applications.
Summary
unavailable
Committee
Closed Committee - Agri-food (AF)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
X - not in an Initiative
Funding Scheme
X – not Funded via a specific Funding Scheme
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