BBSRC Portfolio Analyser
Award details
Isolation of es cells from refractory genotypes by transient ablation of non-es lineages
Reference
D08069
Principal Investigator / Supervisor
Dr James McWhir
Co-Investigators /
Co-Supervisors
Institution
The Roslin Institute
Department
Gene Expression and Development
Funding type
Research
Value (£)
148,281
Status
Completed
Type
Research Grant
Start date
26/05/1997
End date
26/05/2000
Duration
36 months
Abstract
We previously reported the isolation of ES lines from refractory murine genotypes by selective ablation of differentiated cells, demonstrating that a functional ES lineage does exist in these strains. The technique involved explanting embryos from transgenic mice under selection for the expression of an engineered ES-specific selectable marker. Here we seek funding (1) to test the strategy in existing lines of transgenic pig which contain the same transgene, and (2) to further develop the technology for use with embryos derived from non-transgenic donors by direct selection of transfected embryos (transient ablation). Cloning of constructs and characterisation of porcine transgene expression will be done in year 1. Direct selection of transfected murine embryos will be assessed in years 1 and 2, and analogous porcine experiments undertaken in year 3.
Summary
unavailable
Committee
Closed Committee - Agri-food (AF)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
X - not in an Initiative
Funding Scheme
X – not Funded via a specific Funding Scheme
I accept the
terms and conditions of use
(opens in new window)
export PDF file
back to list
new search