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The role of secreted proteins (sips) and their interactions with host cells in virulence of Salmonella

ReferenceD07439
Principal Investigator / Supervisor Dr Edouard Galyov
Co-Investigators /
Co-Supervisors
Institution The Pirbright Institute
DepartmentDiv of Molecular Biology Pirbright
Funding typeResearch
Value (£) 232,397
StatusCompleted
TypeResearch Grant
Start date 06/05/1997
End date 06/05/2000
Duration36 months

Abstract

Salmonella proteins that are likely to be involved in adherence to, and penetration of, host cells and the control of intracellular growth and survival of the bacteria will be investigated. To understand how the disease develops and the methods by which Salmonella can obstruct the primary host defence, the role(s) of S. dublin secreted proteins (Sips) will be assessed. Proposed experiments include: i) Cloning, sequencing, and mutagenesis of S. dublin sip genes ii) Raising anti-Sip antibodies iii) Analysis of Sips activity and localisation in vitro iv) Analysis of the importance of SipB and other Sips for S. dublin virulence in mice v) Analysis of the role of Sip proteins in Salmonella - eukaryotic cell interactions.

Summary

unavailable
Committee Closed Committee - Agri-food (AF)
Research TopicsX – not assigned to a current Research Topic
Research PriorityX – Research Priority information not available
Research Initiative X - not in an Initiative
Funding SchemeX – not Funded via a specific Funding Scheme
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