Award details

Novel DNA diagnostic technology in plant disease control

Reference	D07122
Principal Investigator / Supervisor	Dr Derek Hollomon
Co-Investigators / Co-Supervisors
Institution	Rothamsted Research
Department	Rothamsted Research Department
Funding type	Research
Value (£)	77,592
Status	Completed
Type	Research Grant
Start date	10/03/1997
End date	10/03/2000
Duration	36 months

Abstract

Modern wheat cultivars carry little effective resistance to the important leaf blotch disease, caused by Septoria tritici, and although good fungicides exist their application must be well timed. DNA-based diagnostic systems offer considerable potential to improve leaf blotch control through more accurate pre-symptomatic detection. This project aims to develop generic, microtitre based diagnostic technologies for the detection of plant diseases, using the beta-tubulin gene as the target. It will focus on ways to maximise capture of PCR products in oligonucleotide modified microtire wells, and how to maximise hybridisation of labelled probes to this PCR product. Use of blocking oligonucleotides to hinder reannealing of double stranded PCR product will be explored together with different approaches to generating single stranded PCR products. Application of these technologies as they are developed to measurement of S. triciti in field crops, and incorporation of data into Decision Support Systems, will be carried out in the link programme funded by HGCA.

Summary

unavailable

Committee	Closed Committee - Agri-food (AF)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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