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Award details
Development of transient expression vectors and viral resistance in Agaricus bisporus
Reference
D06309
Principal Investigator / Supervisor
Professor Gary Foster
Co-Investigators /
Co-Supervisors
Professor Peter Mills
Institution
University of Bristol
Department
Biological Sciences
Funding type
Research
Value (£)
223,250
Status
Completed
Type
Research Grant
Start date
01/04/1996
End date
01/04/1999
Duration
36 months
Abstract
The main objective of this proposal is to engineer resistance into Agaricus against viral infection. Resistance mechanisms will be based either directly or indirectly on double-stranded RNA mediated systems. A secondary aim of this project is the generation of a transient viral expression vector, which will allow the high level expression of introduced genes into Agaricus, facilitating the rapid evaluation of introduced genes for crop improvement and manipulation. The project has 4 main aims:- 1) Generation of a full length infectious clone of mushroom bacilliform virus, MBV, 2) Generation of transient expression vectors based on insertion/replacement within MBV infectious plasmid, 3) Effects of 2-5A pathway and dsRNases on levels of virus replication, 4) Agaricus transformation for viral resistance.
Summary
unavailable
Committee
Closed Committee - Agri-food (AF)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
X - not in an Initiative
Funding Scheme
X – not Funded via a specific Funding Scheme
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