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Role of sigma factor E regulated genes in Salmonella pathogenesis and immunity
Reference
D05639
Principal Investigator / Supervisor
Professor Mark Roberts
Co-Investigators /
Co-Supervisors
Institution
University of Glasgow
Department
Veterinary School
Funding type
Research
Value (£)
196,310
Status
Completed
Type
Research Grant
Start date
04/11/1996
End date
04/11/1999
Duration
36 months
Abstract
Sigma factor E (sigma E), is a newly discovered sigma factor that allows the expression of an unknown number of genes in response to stresses that affect the protein content of the outer membrane. The purpose of this work is to investigate the role that sigma E and sigma E-regulated genes play in the Salmonella pathogenesis and immunity. Strains of Salmonella typhimurium will be constructed that do not produce sigma E, produce sigma E constitutively or that cannot negatively regulate sigma E. Mutagenesis with transposons carrying reporter genes will be used to identify and mutate sigma E-regulated genes. The effect of these mutations on the ability of S. typhimurium to survive various insults, including the environment of the phagolysosome of macrophages will be examined. The role of sigma E and sigma E-regulated genes in Salmonella virulence and immunity will be investigated using the murine typhoid model.
Summary
unavailable
Committee
Closed Committee - Agri-food (AF)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
X - not in an Initiative
Funding Scheme
X – not Funded via a specific Funding Scheme
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