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The role of enteroglucagon in the regulation of enterocyte proliferation
Reference
D02537
Principal Investigator / Supervisor
Professor Mohammad Ghatei
Co-Investigators /
Co-Supervisors
Professor Stephen Bloom
,
Dr Robert Goodlad
Institution
Imperial College London
Department
Dept of Medicine
Funding type
Research
Value (£)
106,258
Status
Completed
Type
Research Grant
Start date
01/11/1994
End date
01/11/1997
Duration
36 months
Abstract
Enteroglucagon, a 69 amino acid peptide released from intestinal endocrine cells by food, is elevated when absorption is impaired. We propose it mediates enterocyte growth. To test this hypothesis we will treat primary cultures derived from rat intestinal epithelial cells and two epithelial cell lines, rat IEC6 and human FHs 75 Int, and measure 3H thymidine incorporation and MTT dye uptake. In-vivo, four rat paradigms will be examined, normal feeding, parenteral nutrition, isolated gut loop and partial gut resection and submaximal stimulation by epidermal growth factor. Intestinal growth will be measured by crypt cell production rate, mitotic duration, DNA content and organ weight. Finally enteroglucagon infusions in man will establish the acute effect on enterocyte proliferation.
Summary
unavailable
Committee
Closed Committee - Agri-food (AF)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
X - not in an Initiative
Funding Scheme
X – not Funded via a specific Funding Scheme
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