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Regulated expression of human intestinal dipeptide transporter genes

Reference	D02534
Principal Investigator / Supervisor	Professor Barry Hirst
Co-Investigators / Co-Supervisors	Professor Harry Gilbert, Professor Nicholas Simmons
Institution	Newcastle University
Department	School of Cell and Molecular Biosciences
Funding type	Research
Value (£)	218,876
Status	Completed
Type	Research Grant
Start date	01/11/1994
End date	01/11/1997
Duration	36 months

Abstract

This project aims to test the hypothesis that the spatial and temporal patterns of expression and regulation of the gene products responsible for the absorption of intact peptides from the gut lumen to the blood are controlled by nutrients. Reagents for in situ and antibody studies will be generated. Spatial (duodenal-to-colonic axis) and temporal (crypt-to-villus axis) patterns of peptide transporter mRNA and protein expression will be investigated by in situ hybridisation and by immunohistochemistry in the human small intestine. Human intestinal Caco-2 cells will be used as an in vitro model to study temporal patterns of expression, the role of nutrients on expression (with comparative experiments in mice in vivo) and to delineate possible second messenger systems involved in regulation of peptide transporter gene expression and activity.

Summary

unavailable

Committee	Closed Committee - Agri-food (AF)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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