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Crystallographic studies of dehydroquinate synthase and related structures
Reference
D02454
Principal Investigator / Supervisor
Dr Katherine Brown
Co-Investigators /
Co-Supervisors
Professor Alastair Hawkins
Institution
Imperial College London
Department
Biological Sciences
Funding type
Research
Value (£)
203,067
Status
Completed
Type
Research Grant
Start date
17/10/1994
End date
31/07/1998
Duration
45 months
Abstract
The discovery that chemical disruption of aromatic amino acid biosynthesis can lead to plant death has made this metabolic `shikimate' pathway an attractive target for herbicide development. We have recently obtained crystals of 3-dehydroquinate synthase (DHQS), an essential enzyme in the shikimate pathway, as a complex with a herbicidal compound that diffracts to 1.8 angstroms. We propose to establish a high resolution crystal structure of this enzyme-inhibitor complex. This is expected to lead to a hypothesis about the mode of binding of the inhibitor and substrate; indicate the chemistry of the catalytic mechanism; and aid the design of other useful compounds. In parallel, we will express and purify a bi-functional DHQS/EPSPS enzyme.
Summary
unavailable
Committee
Closed Committee - Agri-food (AF)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
X - not in an Initiative
Funding Scheme
X – not Funded via a specific Funding Scheme
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