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Application of molecular techniques in the quantitative analysis of biological control of Rhizoctonia by Trichoderma
Reference
D02440
Principal Investigator / Supervisor
Professor Christopher Gilligan
Co-Investigators /
Co-Supervisors
Dr Molly Dewey
Institution
University of Cambridge
Department
Plant Sciences
Funding type
Research
Value (£)
93,417
Status
Completed
Type
Research Grant
Start date
01/02/1995
End date
01/05/1997
Duration
27 months
Abstract
The project exploits the application of molecular techniques for the quantification of fungi in soil in order to analyse the epidemiological effectiveness of a hyperparasite as a biocontrol agent. Specifically the objectives are: 1) to apply previously developed and tested monoclonal antibody (mAb) based immunological assays with beta-glucuronidase markers to quantify population cycles of Rhizoctonia solani and Trichoderma harzianum and to determine the relative epidemiological roles of components of hyperparasite biomass in biological control of the pathogen; 2) to use mathematical models to synthesise the population dynamics of R. solani and T. harzianum, obtained from the mAb-based assays, in order to identify critical points in the interaction that would optimise the effectiveness of biocontrol; 3) to use mAb-based assays to analyse the effects and interaction of T. harzianum on the local multiplication of R. solani in the rhizosphere and bulk soil; 4) to continue quantification of R. solani densities in naturally infested soils, using molecular techniques.
Summary
unavailable
Committee
Closed Committee - Agri-food (AF)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
X - not in an Initiative
Funding Scheme
X – not Funded via a specific Funding Scheme
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