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Mechanistic studies on the biosynthesis of glucosinolates in oilseed rape; the aldoxime-forming microsomal enzyme systems
Reference
D02305
Principal Investigator / Supervisor
Dr Nigel Botting
Co-Investigators /
Co-Supervisors
Institution
University of St Andrews
Department
Biology
Funding type
Research
Value (£)
121,148
Status
Completed
Type
Research Grant
Start date
01/01/1995
End date
01/01/1998
Duration
36 months
Abstract
The glucosinolates are an important group of defensive secondary metabolites found in all Crucifers. Currently there is considerable interest in the genetic modification of crucifer crops, especially oilseed rape, to improve their natural crop protection by altering their glucosinolate profile. Such a strategy requires detailed information concerning the biosynthesis of these compounds. They are derived from the precursor amino acids, and the first step in the pathway involves conversion to the corresponding aldoximes. This reaction has recently been demonstrated in microsomes derived from oilseed rape leaves. The aim of the project is to elucidate the mechanism of this interesting and unusual biochemical transformation. A range of techniques including labelling studies, 15N NMR spectroscopy, kinetic studies and the synthesis and evaluation of novel substrate analogues will be employed. The results should allow potential intermediates to be identified and a mechanism to be proposed.
Summary
unavailable
Committee
Closed Committee - Agri-food (AF)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
X - not in an Initiative
Funding Scheme
X – not Funded via a specific Funding Scheme
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